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In this study Anti oxidant activity was performed by DPPH(1,1 diphenyl-2-picryl hydrazyl) radical scavenging method for Tribulus terrestris L. Whole plant methanolic extraction fractionation with toluene and n-butanol in succession. The obtained fractions were concentrated under reduced pressure to yield corresponding antioxidant activity. The IC50 concentration for the standard,ascorbic acid and for BF-TTME were found to be 0.085 and 4.5 ?g/ml respectively.

Polyherbal formulations were developed by using five bioactive N-butanol fractionated extracts of Eclipta alba, Picrorhizakurroa(Rhizome), Terminaliachebula (fruits),Piper nigrum (fruits),Nigella sativa(seeds), Tribulusterristris(whole plant) Linn, treatment of liver disorders by exploiting the knowledge of traditional system of medicine and evaluated for hepatoprotective activity using acute liver toxicity models of paracetamol,ccl4, ethanol, isoniazide induced liver damage in rats. Major active fractions were isolated by solvent fractionation and quantified by column chromatographic method. Two polyherbal tablet formulations were developed by the wet granulation method using microcrystalline cellulose, aerosil and other excipients and subjected for physicochemical evaluation to assess physical stability followed by pharmacological screening. The prepared tablets were finally subjected to stability testing to assess its shelf-life. The rats were monitored for change in liver morphology, biochemical parameters for polyherbal tablet formulation at 50 mg/kg and polyherbal tablet formulation at 100 mg/kg. Both formulations showed significant hepatoprotective activity. The histological studies were also support the biochemical parameters. From the results of biochemical analysis and histopathological studies, Biochemical marker showed improved results for polyherbal tablet formulation at 100 mg/kg. Polyherbal tablet formulation contains a potent hepatoprotective agent suggested to be a butanol fraction concentrated in polyherbal formulation which may find clinical application in amelioration of paracetamol, ethanol, ccl4, isoniazide induced liver damage.